nuclear staining with dapi (Beyotime)
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Nuclear Staining With Dapi, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 32584 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nuclear+staining+with+dapi/DAPI/pm42791620-89-34-39
Average 99 stars, based on 32584 article reviews
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Blocking Assay:Article Title: Integrative network pharmacology, transcriptomics, and microbiomics elucidate the therapeutic mechanism of Polygala tenuifolia Willd water extract in chronic obstructive pulmonary disease Article Snippet: .. Subsequent steps included serum blocking, incubation with a primary antibody (Affinity Biosciences Cat# AF0016), thorough washing, and Incubation:Article Title: Integrative network pharmacology, transcriptomics, and microbiomics elucidate the therapeutic mechanism of Polygala tenuifolia Willd water extract in chronic obstructive pulmonary disease Article Snippet: .. Subsequent steps included serum blocking, incubation with a primary antibody (Affinity Biosciences Cat# AF0016), thorough washing, and Staining:Article Title: Integrative network pharmacology, transcriptomics, and microbiomics elucidate the therapeutic mechanism of Polygala tenuifolia Willd water extract in chronic obstructive pulmonary disease Article Snippet: .. Subsequent steps included serum blocking, incubation with a primary antibody (Affinity Biosciences Cat# AF0016), thorough washing, and Article Title: Hepatocyte Growth Hormone Receptor Ablation Is Associated With Aging Phenotypes and Hepatic Mitochondrial Dysfunction. Article Snippet: Oligomycin (1.5 μM), FCCP (1.0 μM), and rotenone/antimycin A (0.5 μM) were sequentially injected, and key parameters, including non- mitochondrial oxygen consumption, basal respiration, maximal respiration, ATP production, and spare respiratory capacity, were analyzed using Wave software (Agilent). .. After reaching about 70% confluence in confocal dishes, cells were stained with Mito- Tracker (1 mM, C1997S, Beyotime Biotechnology, China) or BODIPY 493/503 (1 μg/mL, C2053S, Beyotime Biotechnology, China) for 30 min, followed by Article Title: TRIM59 alleviates neuronal ferroptosis and promotes functional recovery after spinal cord injury by mediating ubiquitination and degradation of ANXA2 Article Snippet: .. Subsequently, the sections received secondary antibodies (Alexa Fluor 488 or Alexa Fluor 594 conjugates; 1:200; Shanghai Fushen Biotechnology) for 60 min at ambient temperature, then underwent Article Title: Induction of notochordal differentiation of bone marrow mesenchymal-derived stem cells via the stimulation of notochordal cell-rich nucleus pulposus tissue Article Snippet: The cells were then incubated with Alexa Fluor ® 555-conjugated goat-derived IgG secondary antibody (1:5,000; cat. no. 95235; Cell Signaling Technology, Inc.) for 1 h at 37°C in the dark. .. Article Title: The role of ancient ubiquitous protein 1 (Aup1) in regulating hepatic lipid droplet levels, endoplasmic reticulum stress, and inflammation in zebrafish (Danio rerio). Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Article Title: Borneol-grafted poly(amidoamine) dendrimer with dual antimicrobial and enamel-remineralizing activities against early-stage dental caries. Article Snippet: For cytotoxicity screening, HOKs were exposed to BN-PAMAM (0.5–4 mg/mL) for 24 h. Cell viability was evaluated using a Cell Counting Kit-8 (CCK-8; Beyotime Biotechnology (Beyotime), Shanghai, China) at 450 nm. .. For comparative testing, HOKs were treated with BN-PAMAM, PAMAM, or BN (2 mg/ mL), or controls, for 24 h. Live/Dead staining (Thermo Fisher), F-actin staining with phalloidin (Beyotime), and Confocal:Article Title: Hepatocyte Growth Hormone Receptor Ablation Is Associated With Aging Phenotypes and Hepatic Mitochondrial Dysfunction. Article Snippet: Oligomycin (1.5 μM), FCCP (1.0 μM), and rotenone/antimycin A (0.5 μM) were sequentially injected, and key parameters, including non- mitochondrial oxygen consumption, basal respiration, maximal respiration, ATP production, and spare respiratory capacity, were analyzed using Wave software (Agilent). .. After reaching about 70% confluence in confocal dishes, cells were stained with Mito- Tracker (1 mM, C1997S, Beyotime Biotechnology, China) or BODIPY 493/503 (1 μg/mL, C2053S, Beyotime Biotechnology, China) for 30 min, followed by Fluorescence:Article Title: Induction of notochordal differentiation of bone marrow mesenchymal-derived stem cells via the stimulation of notochordal cell-rich nucleus pulposus tissue Article Snippet: The cells were then incubated with Alexa Fluor ® 555-conjugated goat-derived IgG secondary antibody (1:5,000; cat. no. 95235; Cell Signaling Technology, Inc.) for 1 h at 37°C in the dark. .. Article Title: Borneol-grafted poly(amidoamine) dendrimer with dual antimicrobial and enamel-remineralizing activities against early-stage dental caries. Article Snippet: For cytotoxicity screening, HOKs were exposed to BN-PAMAM (0.5–4 mg/mL) for 24 h. Cell viability was evaluated using a Cell Counting Kit-8 (CCK-8; Beyotime Biotechnology (Beyotime), Shanghai, China) at 450 nm. .. For comparative testing, HOKs were treated with BN-PAMAM, PAMAM, or BN (2 mg/ mL), or controls, for 24 h. Live/Dead staining (Thermo Fisher), F-actin staining with phalloidin (Beyotime), and Microscopy:Article Title: Induction of notochordal differentiation of bone marrow mesenchymal-derived stem cells via the stimulation of notochordal cell-rich nucleus pulposus tissue Article Snippet: The cells were then incubated with Alexa Fluor ® 555-conjugated goat-derived IgG secondary antibody (1:5,000; cat. no. 95235; Cell Signaling Technology, Inc.) for 1 h at 37°C in the dark. .. Article Title: Borneol-grafted poly(amidoamine) dendrimer with dual antimicrobial and enamel-remineralizing activities against early-stage dental caries. Article Snippet: For cytotoxicity screening, HOKs were exposed to BN-PAMAM (0.5–4 mg/mL) for 24 h. Cell viability was evaluated using a Cell Counting Kit-8 (CCK-8; Beyotime Biotechnology (Beyotime), Shanghai, China) at 450 nm. .. For comparative testing, HOKs were treated with BN-PAMAM, PAMAM, or BN (2 mg/ mL), or controls, for 24 h. Live/Dead staining (Thermo Fisher), F-actin staining with phalloidin (Beyotime), and |
