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nuclear staining with dapi  (Beyotime)


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    Structured Review

    Beyotime nuclear staining with dapi
    Nuclear Staining With Dapi, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 32584 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nuclear+staining+with+dapi/DAPI/pm42791620-89-34-39
    Average 99 stars, based on 32584 article reviews
    nuclear staining with dapi - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Blocking Assay:

    Article Title: Integrative network pharmacology, transcriptomics, and microbiomics elucidate the therapeutic mechanism of Polygala tenuifolia Willd water extract in chronic obstructive pulmonary disease
    Article Snippet: .. Subsequent steps included serum blocking, incubation with a primary antibody (Affinity Biosciences Cat# AF0016), thorough washing, and nuclear staining with DAPI (Beyotime, China). ..

    Incubation:

    Article Title: Integrative network pharmacology, transcriptomics, and microbiomics elucidate the therapeutic mechanism of Polygala tenuifolia Willd water extract in chronic obstructive pulmonary disease
    Article Snippet: .. Subsequent steps included serum blocking, incubation with a primary antibody (Affinity Biosciences Cat# AF0016), thorough washing, and nuclear staining with DAPI (Beyotime, China). ..

    Staining:

    Article Title: Integrative network pharmacology, transcriptomics, and microbiomics elucidate the therapeutic mechanism of Polygala tenuifolia Willd water extract in chronic obstructive pulmonary disease
    Article Snippet: .. Subsequent steps included serum blocking, incubation with a primary antibody (Affinity Biosciences Cat# AF0016), thorough washing, and nuclear staining with DAPI (Beyotime, China). ..

    Article Title: Hepatocyte Growth Hormone Receptor Ablation Is Associated With Aging Phenotypes and Hepatic Mitochondrial Dysfunction.
    Article Snippet: Oligomycin (1.5 μM), FCCP (1.0 μM), and rotenone/antimycin A (0.5 μM) were sequentially injected, and key parameters, including non- mitochondrial oxygen consumption, basal respiration, maximal respiration, ATP production, and spare respiratory capacity, were analyzed using Wave software (Agilent). .. After reaching about 70% confluence in confocal dishes, cells were stained with Mito- Tracker (1 mM, C1997S, Beyotime Biotechnology, China) or BODIPY 493/503 (1 μg/mL, C2053S, Beyotime Biotechnology, China) for 30 min, followed by nuclear staining with DAPI (P0131, Beyotime Biotechnology, China). ..

    Article Title: TRIM59 alleviates neuronal ferroptosis and promotes functional recovery after spinal cord injury by mediating ubiquitination and degradation of ANXA2
    Article Snippet: .. Subsequently, the sections received secondary antibodies (Alexa Fluor 488 or Alexa Fluor 594 conjugates; 1:200; Shanghai Fushen Biotechnology) for 60 min at ambient temperature, then underwent nuclear staining with DAPI (Beyotime, China). .. A fluorescence microscope was utilized to capture images, and ImageJ software facilitated the quantitative assessment.

    Article Title: Induction of notochordal differentiation of bone marrow mesenchymal-derived stem cells via the stimulation of notochordal cell-rich nucleus pulposus tissue
    Article Snippet: The cells were then incubated with Alexa Fluor ® 555-conjugated goat-derived IgG secondary antibody (1:5,000; cat. no. 95235; Cell Signaling Technology, Inc.) for 1 h at 37°C in the dark. .. Nuclear staining with DAPI (Beyotime Institute of Biotechnology) was conducted at 37°C for 3 min, and results were observed under a fluorescence microscope (magnification, ×200; Olympus IX50; Olympus Corporation). ..

    Article Title: The role of ancient ubiquitous protein 1 (Aup1) in regulating hepatic lipid droplet levels, endoplasmic reticulum stress, and inflammation in zebrafish (Danio rerio).
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Article Title: Borneol-grafted poly(amidoamine) dendrimer with dual antimicrobial and enamel-remineralizing activities against early-stage dental caries.
    Article Snippet: For cytotoxicity screening, HOKs were exposed to BN-PAMAM (0.5–4 mg/mL) for 24 h. Cell viability was evaluated using a Cell Counting Kit-8 (CCK-8; Beyotime Biotechnology (Beyotime), Shanghai, China) at 450 nm. .. For comparative testing, HOKs were treated with BN-PAMAM, PAMAM, or BN (2 mg/ mL), or controls, for 24 h. Live/Dead staining (Thermo Fisher), F-actin staining with phalloidin (Beyotime), and nuclear staining with DAPI (Beyotime) were imaged under inverted fluorescence microscopy (IX73, Olympus, Tokyo, Japan). ..

    Confocal:

    Article Title: Hepatocyte Growth Hormone Receptor Ablation Is Associated With Aging Phenotypes and Hepatic Mitochondrial Dysfunction.
    Article Snippet: Oligomycin (1.5 μM), FCCP (1.0 μM), and rotenone/antimycin A (0.5 μM) were sequentially injected, and key parameters, including non- mitochondrial oxygen consumption, basal respiration, maximal respiration, ATP production, and spare respiratory capacity, were analyzed using Wave software (Agilent). .. After reaching about 70% confluence in confocal dishes, cells were stained with Mito- Tracker (1 mM, C1997S, Beyotime Biotechnology, China) or BODIPY 493/503 (1 μg/mL, C2053S, Beyotime Biotechnology, China) for 30 min, followed by nuclear staining with DAPI (P0131, Beyotime Biotechnology, China). ..

    Fluorescence:

    Article Title: Induction of notochordal differentiation of bone marrow mesenchymal-derived stem cells via the stimulation of notochordal cell-rich nucleus pulposus tissue
    Article Snippet: The cells were then incubated with Alexa Fluor ® 555-conjugated goat-derived IgG secondary antibody (1:5,000; cat. no. 95235; Cell Signaling Technology, Inc.) for 1 h at 37°C in the dark. .. Nuclear staining with DAPI (Beyotime Institute of Biotechnology) was conducted at 37°C for 3 min, and results were observed under a fluorescence microscope (magnification, ×200; Olympus IX50; Olympus Corporation). ..

    Article Title: Borneol-grafted poly(amidoamine) dendrimer with dual antimicrobial and enamel-remineralizing activities against early-stage dental caries.
    Article Snippet: For cytotoxicity screening, HOKs were exposed to BN-PAMAM (0.5–4 mg/mL) for 24 h. Cell viability was evaluated using a Cell Counting Kit-8 (CCK-8; Beyotime Biotechnology (Beyotime), Shanghai, China) at 450 nm. .. For comparative testing, HOKs were treated with BN-PAMAM, PAMAM, or BN (2 mg/ mL), or controls, for 24 h. Live/Dead staining (Thermo Fisher), F-actin staining with phalloidin (Beyotime), and nuclear staining with DAPI (Beyotime) were imaged under inverted fluorescence microscopy (IX73, Olympus, Tokyo, Japan). ..

    Microscopy:

    Article Title: Induction of notochordal differentiation of bone marrow mesenchymal-derived stem cells via the stimulation of notochordal cell-rich nucleus pulposus tissue
    Article Snippet: The cells were then incubated with Alexa Fluor ® 555-conjugated goat-derived IgG secondary antibody (1:5,000; cat. no. 95235; Cell Signaling Technology, Inc.) for 1 h at 37°C in the dark. .. Nuclear staining with DAPI (Beyotime Institute of Biotechnology) was conducted at 37°C for 3 min, and results were observed under a fluorescence microscope (magnification, ×200; Olympus IX50; Olympus Corporation). ..

    Article Title: Borneol-grafted poly(amidoamine) dendrimer with dual antimicrobial and enamel-remineralizing activities against early-stage dental caries.
    Article Snippet: For cytotoxicity screening, HOKs were exposed to BN-PAMAM (0.5–4 mg/mL) for 24 h. Cell viability was evaluated using a Cell Counting Kit-8 (CCK-8; Beyotime Biotechnology (Beyotime), Shanghai, China) at 450 nm. .. For comparative testing, HOKs were treated with BN-PAMAM, PAMAM, or BN (2 mg/ mL), or controls, for 24 h. Live/Dead staining (Thermo Fisher), F-actin staining with phalloidin (Beyotime), and nuclear staining with DAPI (Beyotime) were imaged under inverted fluorescence microscopy (IX73, Olympus, Tokyo, Japan). ..



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    (A) Schematic representation of an OrganoPlate® 3-lane tissue chip formed by two channels for cell seeding and a middle channel for gel loading. (B) Brightfield image and (C) confocal projection of HUVECs (red) seeded on top channel and HDLECs (green) seeded in bottom channel. Images taken after 24 h of culture under flow conditions. (D) Representative confocal max projection of HDLECs cultured in flow for 14 days in OrganoPlate® and treated for 24 h with DENV-2 NS1 (NS1) or control medium (untreated=Unt). Nuclei stained with <t>DAPI.</t> Merged images shows VE-cadherin (green) and F-actin (red) and DAPI (blue). Scale bar = 30 µm.
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    Beyotime dapi nuclear stain
    (A) Schematic representation of an OrganoPlate® 3-lane tissue chip formed by two channels for cell seeding and a middle channel for gel loading. (B) Brightfield image and (C) confocal projection of HUVECs (red) seeded on top channel and HDLECs (green) seeded in bottom channel. Images taken after 24 h of culture under flow conditions. (D) Representative confocal max projection of HDLECs cultured in flow for 14 days in OrganoPlate® and treated for 24 h with DENV-2 NS1 (NS1) or control medium (untreated=Unt). Nuclei stained with <t>DAPI.</t> Merged images shows VE-cadherin (green) and F-actin (red) and DAPI (blue). Scale bar = 30 µm.
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    Servicebio Inc dapi nuclear staining
    (A) Schematic representation of an OrganoPlate® 3-lane tissue chip formed by two channels for cell seeding and a middle channel for gel loading. (B) Brightfield image and (C) confocal projection of HUVECs (red) seeded on top channel and HDLECs (green) seeded in bottom channel. Images taken after 24 h of culture under flow conditions. (D) Representative confocal max projection of HDLECs cultured in flow for 14 days in OrganoPlate® and treated for 24 h with DENV-2 NS1 (NS1) or control medium (untreated=Unt). Nuclei stained with <t>DAPI.</t> Merged images shows VE-cadherin (green) and F-actin (red) and DAPI (blue). Scale bar = 30 µm.
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    Cell Signaling Technology Inc dapi nuclear stain
    (A) Schematic representation of an OrganoPlate® 3-lane tissue chip formed by two channels for cell seeding and a middle channel for gel loading. (B) Brightfield image and (C) confocal projection of HUVECs (red) seeded on top channel and HDLECs (green) seeded in bottom channel. Images taken after 24 h of culture under flow conditions. (D) Representative confocal max projection of HDLECs cultured in flow for 14 days in OrganoPlate® and treated for 24 h with DENV-2 NS1 (NS1) or control medium (untreated=Unt). Nuclei stained with <t>DAPI.</t> Merged images shows VE-cadherin (green) and F-actin (red) and DAPI (blue). Scale bar = 30 µm.
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    Image Search Results


    (A) Schematic representation of an OrganoPlate® 3-lane tissue chip formed by two channels for cell seeding and a middle channel for gel loading. (B) Brightfield image and (C) confocal projection of HUVECs (red) seeded on top channel and HDLECs (green) seeded in bottom channel. Images taken after 24 h of culture under flow conditions. (D) Representative confocal max projection of HDLECs cultured in flow for 14 days in OrganoPlate® and treated for 24 h with DENV-2 NS1 (NS1) or control medium (untreated=Unt). Nuclei stained with DAPI. Merged images shows VE-cadherin (green) and F-actin (red) and DAPI (blue). Scale bar = 30 µm.

    Journal: bioRxiv

    Article Title: Lymphatic vessel dysfunction contributes to severe dengue pathogenesis

    doi: 10.64898/2026.03.27.714698

    Figure Lengend Snippet: (A) Schematic representation of an OrganoPlate® 3-lane tissue chip formed by two channels for cell seeding and a middle channel for gel loading. (B) Brightfield image and (C) confocal projection of HUVECs (red) seeded on top channel and HDLECs (green) seeded in bottom channel. Images taken after 24 h of culture under flow conditions. (D) Representative confocal max projection of HDLECs cultured in flow for 14 days in OrganoPlate® and treated for 24 h with DENV-2 NS1 (NS1) or control medium (untreated=Unt). Nuclei stained with DAPI. Merged images shows VE-cadherin (green) and F-actin (red) and DAPI (blue). Scale bar = 30 µm.

    Article Snippet: The slides were then incubated with the nuclear stain DAPI (cat# D9542, Merck) at 1:1000 dilution for 10 min and mounted onto glass slides using mounting medium (InvitrogenTM Fluoromount-GTM Mounting Medium-00495802).

    Techniques: Cell Culture, Control, Staining